Rapid communication: an XbaI restriction fragment length polymorphism at the Porcine T-complex 1 (TCP1) locus.

Polymorphism. A restriction fragment length polymorphism (RFLP) was detected in the swine TCPl locus using the restriction enzyme XbaI. Source and Description of Clone. A 580-bp human cDNA clone for TCPl was excised from the EcoRI site of plasmid pB1.4 hum (Willison et al., 1987). Method of Detection. DNA was isolated from whole blood, digested with XbaI, separated by agarose gel electrophoresis, and transferred to charged nylon membranes. Hybridizations were performed at 65°C for 16 to 20 h in 10% dextran sulfate, .5 M NaC1, .05 M sodium phosphate, pH 6.5, .5% SDS, and 100 pg/mL of sonicated, denatured salmon sperm DNA. Final washes were at 58°C in .7x SSC, 5% SDS for 15 min. Description of Polymorphism. Hybridization of XbaI digests revealed four fragments (Figure 1). The 9.0-kb and 3.5-kb fragments were monomorphic, and the 12.4-kb and 11.2-kb fragments were polymorphic. TCPl polymorphisms were also detected with SstI (36 animals). Inheritance Pattern. Autosomal Mendelian segregation of the 12.4-kb and 11.2-kb XbaI fragment bands